30 Day Money Back Guarantee and Free Shipping on All Contiguous US Orders
Free Shipping on All Contiguous US Orders

Image caption appears here

Methylene blue improves sensorimotor phenotype and decreases anxiety in parallel with activating brain mitochondria biogenesis in mid-age mice

    Methylene blue improves sensorimotor phenotype and decreases anxiety in parallel with activating brain mitochondria biogenesis in mid-age mice

    Artem P Gureev et al. Neurosci Res. 2016 Dec.


    Age-related brain dysfunctions are associated with mitochondria malfunctions and increased risk of developing neurodegenerative diseases (ND). Recently, a mitochondria-targeting drug methylene blue has been drawing considerable interest as a potential treatment for ND. We found that aged mice manifested a decrease in physical endurance, spontaneous locomotor activity, and exploration concomitant with an increase in anxiety-related behavior, as compared to adult mice. Treating mice for 60 days with MB slowed down these changes. There were no significant changes in the animals' body weight, oxygen consumption rates, or respiratory quotient index, in adult or aged MB-treated mice. However, MB treatment significantly increased the generation of reactive oxygen species in brain mitochondria. The expression of several genes relevant to mitochondria biogenesis, bioenergetics, and antioxidant defense (NRF1, MTCOX1, TFAM, and SOD2) was greatly suppressed in aged mice; it was restored by MB treatment. It seems plausible that the effects of MB could be mediated by its ability to increase H2O2 production in brain mitochondria, thereby activating Nrf2/ARE signaling pathway and mitochondria biogenesis. Our data and earlier findings support the idea that MB can be an attractive prototype drug for developing safe and efficient gerontoprotective compounds.

    Keywords: Aging; Behavior; Hydrogen peroxide; Methylene blue; Mitochondria.

    Similar articles

    Cited by

    MeSH terms

    Related Articles

    Understanding the Proper Dosing of Methylene Blue: A Comprehensive Guide

    In this comprehensive guide on dosing methylene blue, several key points are highlighted. It emphasizes the importance of consulting with a healthcare professional before starting methylene blue supplementation. The purpose and formulation of methylene blue should be considered, as the dosage can vary based on its intended use. Starting with low doses and gradually increasing as tolerated is recommended to assess individual sensitivity and minimize adverse effects. Understanding measurement units and adjusting the dosage based on individual factors such as body weight, age, metabolism, and specific health conditions is crucial. Adhering to recommended guidelines and seeking professional guidance throughout the process are essential for safe and effective usage. By following these principles, individuals can make informed decisions and optimize the benefits of methylene blue while minimizing potential risks.


    Group 23